Quantification of apolipoprotein B-48 and B-100 in rat liver endoplasmic reticulum and Golgi fractions
نویسندگان
چکیده
منابع مشابه
Apolipoprotein B 100 and 48
Apolipoprotein B is the main structural surface protein found on all beta-lipoproteins (Chylomicrons, VLDLs, IDLs and LDLs). There is a single molecule of apoB on each of those lipoproteins. It is the only apolipoprotein that is not transferablei.e. it is with the particle from its birth till its death. Beta-lipoproteins are the lipoproteins capable of trafficking cholesterol into the artery wa...
متن کاملGeneration of monoclonal antibodies specific for mouse apolipoprotein B-100 in apolipoprotein B-48-only mice.
Over the past 10 years, many laboratories have investigated lipid metabolism and atherogenesis with a variety of transgenic and gene knockout mouse models. Although many of these studies have yielded valuable insights, some have been hampered by a paucity of useful antibodies against mouse proteins. For example, many laboratories have analyzed genetic and dietary interventions affecting lipopro...
متن کاملApolipoprotein B-100 deficiency. Intestinal steatosis despite apolipoprotein B-48 synthesis.
We describe a child, the issue of phenotypically normal parents, who had fat malabsorption, both intestinal and hepatic steatosis, and serum cholesterol and triglyceride concentrations of 38 and 63 mg/dl, respectively. Lipoprotein electrophoresis, Ouchterlony double diffusion, and electron microscopy demonstrated that normal low density lipoproteins (LDL: 1.006 less than rho less than 1.063 g/m...
متن کاملA quantitative immunoassay for apolipoprotein B in plasma lipoproteins and subcellular fractions of rat liver.
HDL in the coronary survivors suggests an impairment in the process of reverse cholesterol transport in which this fraction has been demonstrated to be implicated in animal studies [8]. The results of this study therefore suggest that measurements of these HDL subfractions may provide better indicators of coronary risk than that of total plasma cholesterol which is frequently employed for this ...
متن کاملTwo fractions of rough endoplasmic reticulum from rat liver. I. Recovery of rapidly sedimenting endoplasmic reticulum in association with mitochondria
Low-speed centrifugation (640 g) of rat liver homogenates, prepared with a standard ionic medium, yielded a pellet from which a rapidly sedimenting fraction of rough endoplasmic reticulum (RSER) was recovered free of nuclei. This fraction contained 20-25% of cellular RNA and approximately 30% of total glucose-6-phosphatase (ER marker) activity. A major portion of total cytochrome c oxidase (mit...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1992
ISSN: 0264-6021,1470-8728
DOI: 10.1042/bj2850153